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Microfluidics is a quickly growing, highly interdisciplinary field at the interface of physics, engineering, chemistry and biology. The EMBL Microfluidics Conference 2020 aims to bring together top researchers in the field and to spark scientific exchange, also across different disciplines. The latest Lab-on-a-Chip technologies and applications will be presented, which should be of major interest for experts as well as scientists looking for a first glance at this exciting new technology.